Nitro Blue Tetrazolium
Stand the fundamental chemistry of the nitroblue tetrazolium nbt reaction that lies at the heart of this analysis.
Nitro blue tetrazolium. To measure superoxide radicals. Nitro blue tetrazolium chloride nbt molecular weight. Bacterial killing assays using a patient s neutrophils with either the patient s or control serum and bacteria such as staphylococcus aureus or escherichia coli are a definitive method to determine whether a given patient s neutrophils have an intracellular killing defect.
Nitroblue tetrazolium c40h30n10o6 2 cid 9282 structure chemical names physical and chemical properties classification patents literature biological activities safety hazards toxicity information supplier lists and more. Nitro blue tetrazolium is a tetrazole compound with two tetrazole rings. How the test is performed a blood sample is needed.
The chemical nbt is added to the white blood cells in the lab. Nitro blue tetrazolium is a chemical compound composed of two tetrazole moieties. While it is true that superoxide anion will give up an electron to reduce nbt in truth any enzyme capable of effecting nbt reduction using for.
The nitroblue tetrazolium nbt test is still used for rapid assessment of the respiratory burst when flow cytometry is not available. The tetrazoinyl radical is generated by interaction with superoxide and disruption of the tetrazole ring which subsequently dismutates to generate the bright blue stable formazan product. In lactate dehydrogenase quantification assay.
It is used in immunology for sensitive detection of alkaline phosphatase with bcip. Nitroblue tetrazolium nbt is pale yellow in color prior to reduction by superoxide. The nitroblue tetrazolium test checks if certain immune system cells can change a colorless chemical called nitroblue tetrazolium nbt into a deep blue color.
Nbt serves as the oxidant and bcip is the ap substrate and gives also dark blue dye. Nitro blue tetrazolium has been used.
