Nitro Blue Tetrazolium Test
How the test is performed.
Nitro blue tetrazolium test. Nitro blue tetrazolium is used in a diagnostic test particularly for chronic granulomatous disease and other diseases of phagocyte function. Stand the fundamental chemistry of the nitroblue tetrazolium nbt reaction that lies at the heart of this analysis. The reduction of nitroblue tetrazolium nbt dye by neutrophils from 379 patients with infectious diseases and 268 controls has been examined.
Nitroblue tetrazolium n b t is an interesting compound which is of value in the study of neutrophil physiology. Nbt becomes reduced by free oxygen radicals forming a blue black compound formazan. The technique is simple and inexpensive and requires only a few microlitres of blood.
The chemical nbt is added to the white blood cells in the lab. The nitroblue tetrazolium test checks if certain immune system cells can change a colorless chemical called nitroblue tetrazolium nbt into a deep blue color. Nbt will undergo re duction to form an insoluble blue black product formazan.
How the test is performed a blood sample is needed. The mean nbt score was 29 8 72 3 positive tests in the 231 patients with non tuberculous bacterial infections 9 7 28 1 positive tests in the 135 patients with viral infections 5 3 1 5 positive tests in the controls. As a result bacteria may thrive within the phagocyte.
Nitroblue tetrazolium test or nbt test measures ros generated by both spermatozoa and leukocytes. When there is an nadph oxidase defect the phagocyte is unable to make reactive oxygen species or radicals required for bacterial killing. Chronic granulomatous disease cgd in utero has recently been detected by a new qualitative nitro blue tetrazolium nbt reduction slide test using phorbol myristate acetate pma as a stimulus.
The chemical nbt is added to the white blood cells in the lab. The nitroblue tetrazolium test checks if certain immune system cells can change a colorless chemical called nitroblue tetrazolium nbt into a deep blue color.
